enterococcus faecalis ef atcc 29212 Search Results


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ATCC e faecalis
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ATCC enterococcus faecalis atcc 29212
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ATCC e faecalis atcc 29212
E Faecalis Atcc 29212, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ATCC e faecalis atcc 51299
Experimental workflow for iTRAQ labeling and analysis: iTRAQ 8-plex labeling was performed for two sets of biological replicates of the strains Ef 63, Ef 64, ATCC 51299, and ATCC 29212. Each strain was labeled as follows for the two biological replicates: Ef 63 (113, 117), Ef 64 (114, 118), <t>ATCC</t> <t>51299</t> (115, 119), and ATCC 29212 (116, 121). The labeled fractions were combined and subjected to strong cation exchange chromatography and desalting, followed by separation using liquid chromatography mass spectrometry (LC-MS/MS), and bioinformatics data analysis.
E Faecalis Atcc 51299, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Experimental workflow for iTRAQ labeling and analysis: iTRAQ 8-plex labeling was performed for two sets of biological replicates of the strains Ef 63, Ef 64, ATCC 51299, and ATCC 29212. Each strain was labeled as follows for the two biological replicates: Ef 63 (113, 117), Ef 64 (114, 118), ATCC 51299 (115, 119), and ATCC 29212 (116, 121). The labeled fractions were combined and subjected to strong cation exchange chromatography and desalting, followed by separation using liquid chromatography mass spectrometry (LC-MS/MS), and bioinformatics data analysis.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation *

doi: 10.1074/mcp.RA117.000461

Figure Lengend Snippet: Experimental workflow for iTRAQ labeling and analysis: iTRAQ 8-plex labeling was performed for two sets of biological replicates of the strains Ef 63, Ef 64, ATCC 51299, and ATCC 29212. Each strain was labeled as follows for the two biological replicates: Ef 63 (113, 117), Ef 64 (114, 118), ATCC 51299 (115, 119), and ATCC 29212 (116, 121). The labeled fractions were combined and subjected to strong cation exchange chromatography and desalting, followed by separation using liquid chromatography mass spectrometry (LC-MS/MS), and bioinformatics data analysis.

Article Snippet: Standard laboratory strains E. faecalis ATCC 29212 and E. faecalis ATCC 51299 were used as the control strains.

Techniques: Labeling, Chromatography, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

Determination of experimental variation for the identified proteins: (A) experimental variation of Ef 63 compared with ATCC 29212; (B) experimental variation of Ef 64 compared with ATCC 29212; (C) experimental variation of Ef 63 compared with ATCC 51299; and (D) experimental variation of Ef 64 compared with ATCC 51299. The horizontal axis represents % variation of iTRAQ ratios. The primary vertical axis represents the corresponding number of proteins (bars) having different % variation. The secondary vertical axis represents the cumulative % of the counted proteins. Variation against 88% coverage of population was considered as the cutoff value.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation *

doi: 10.1074/mcp.RA117.000461

Figure Lengend Snippet: Determination of experimental variation for the identified proteins: (A) experimental variation of Ef 63 compared with ATCC 29212; (B) experimental variation of Ef 64 compared with ATCC 29212; (C) experimental variation of Ef 63 compared with ATCC 51299; and (D) experimental variation of Ef 64 compared with ATCC 51299. The horizontal axis represents % variation of iTRAQ ratios. The primary vertical axis represents the corresponding number of proteins (bars) having different % variation. The secondary vertical axis represents the cumulative % of the counted proteins. Variation against 88% coverage of population was considered as the cutoff value.

Article Snippet: Standard laboratory strains E. faecalis ATCC 29212 and E. faecalis ATCC 51299 were used as the control strains.

Techniques:

Volcano plots of differentially regulated proteins reveal significance patterns: (A) volcano plots of differentially regulated proteins of Ef 63 compared with ATCC 29212; (B) volcano plots of differentially regulated proteins of Ef 64 compared with ATCC 29212; (C) volcano plots of differentially regulated proteins of Ef 63 compared with ATCC 51299; and (D) volcano plots of differentially regulated proteins of Ef 64 compared with ATCC 51299. Threshold cutoffs determined for log2 fold change ratios are represented by green lines. p value cutoff of 0.05 is represented by a yellow line. Gray dots represent proteins that do not show significant differences in expression, yellow dots represent proteins that show significant differences in expression but within the cutoff log2 fold change ratios, and red dots represent proteins that show significant differences in expression outside the cut-off log2 fold change ratios.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Proteomics of Strong and Weak Biofilm Formers of Enterococcus faecalis Reveals Novel Regulators of Biofilm Formation *

doi: 10.1074/mcp.RA117.000461

Figure Lengend Snippet: Volcano plots of differentially regulated proteins reveal significance patterns: (A) volcano plots of differentially regulated proteins of Ef 63 compared with ATCC 29212; (B) volcano plots of differentially regulated proteins of Ef 64 compared with ATCC 29212; (C) volcano plots of differentially regulated proteins of Ef 63 compared with ATCC 51299; and (D) volcano plots of differentially regulated proteins of Ef 64 compared with ATCC 51299. Threshold cutoffs determined for log2 fold change ratios are represented by green lines. p value cutoff of 0.05 is represented by a yellow line. Gray dots represent proteins that do not show significant differences in expression, yellow dots represent proteins that show significant differences in expression but within the cutoff log2 fold change ratios, and red dots represent proteins that show significant differences in expression outside the cut-off log2 fold change ratios.

Article Snippet: Standard laboratory strains E. faecalis ATCC 29212 and E. faecalis ATCC 51299 were used as the control strains.

Techniques: Expressing